【文獻(xiàn)標(biāo)題】Hepatic Stellate Cells Secreted Hepatocyte Growth Factor Contributes to the Chemoresistance of Hepatocellular Carcinoma

【作者】Guofeng Yu, Yingying Jing, Xingrui Kou, et.al

【作者單位】上海第二軍醫(yī)大學(xué)（Second Military Medical University, Shanghai）

【文獻(xiàn)中引用產(chǎn)品】

人肝細(xì)胞生長(zhǎng)因子(HGF)ELISA試劑盒

人轉(zhuǎn)化生長(zhǎng)因子β（TGF-β）ELISA試劑盒

【DOI】doi:10.1371/journal.pone.0073312

【影響因子(IF)】3.23

【出版期刊】《PLOS ONE》

【產(chǎn)品原文引用】

Conditioned medium and ELISA

Cultured media (CM) were collected from LX-2 cells cultured in 2D culture dishes and centrifuged at 1000g for 5 minutes to obtain the supernatant. LX-2 CM was incubated with HGF antibody (GTX10678, 0.5 μg/ml) for 24h at 4oC to obtain HGFdepleted LX-2 CM. The level of HGF and TGF-β in CM waere determined by using human HGF ELISA kit (HY10158E, Shanghai HengYuan Biological Technology Co., Ltd) and TGF- β ELISA kit (HY10108E, Shanghai HengYuan Biological Technology Co., Ltd) according to manufacturer’s instructions.

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